Ultrafast video imaging of cell division from zebrafish egg using multimodal microscopic system

Sung-Ho Lee; Bumjoon Jang; Dong Hee Kim; Chang Hyun Park; Gyuri Bae; Seung Woo Park; Seung-Han Park

doi:10.1117/12.2270692

12 July 2017 Ultrafast video imaging of cell division from zebrafish egg using multimodal microscopic system

Sung-Ho Lee, Bumjoon Jang, Dong Hee Kim, Chang Hyun Park, Gyuri Bae, Seung Woo Park, Seung-Han Park

Proceedings Volume 10324, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017; 103240B (2017) https://doi.org/10.1117/12.2270692
Event: International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017, 2017, Jeju, Korea, Republic of

Abstract

Unlike those of other ordinary laser scanning microscopies in the past, nonlinear optical laser scanning microscopy (SHG, THG microscopy) applied ultrafast laser technology which has high peak powers with relatively inexpensive, low-average-power. It short pulse nature reduces the ionization damage in organic molecules. And it enables us to take bright label-free images. In this study, we measured cell division of zebrafish egg with ultrafast video images using multimodal nonlinear optical microscope. The result shows in-vivo cell division label-free imaging with sub-cellular resolution.

Citation Download Citation

Sung-Ho Lee, Bumjoon Jang, Dong Hee Kim, Chang Hyun Park, Gyuri Bae, Seung Woo Park, and Seung-Han Park "Ultrafast video imaging of cell division from zebrafish egg using multimodal microscopic system", Proc. SPIE 10324, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017, 103240B (12 July 2017); https://doi.org/10.1117/12.2270692

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KEYWORDS

Ultrafast imaging

Imaging systems

Microscopy

Ionization

Laser applications

Optical microscopy

Second-harmonic generation

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