12 July 2017 Multimodal nonlinear imaging of arabidopsis thaliana root cell
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Proceedings Volume 10324, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017; 103240C (2017) https://doi.org/10.1117/12.2270697
Event: International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017, 2017, Jeju, Korea, Republic of
Abstract
Nonlinear optical microscopy has enabled the possibility to explore inside the living organisms. It utilizes ultrashort laser pulse with long wavelength (greater than 800nm). Ultrashort pulse produces high peak power to induce nonlinear optical phenomenon such as two-photon excitation fluorescence (TPEF) and harmonic generations in the medium while maintaining relatively low average energy pre area. In plant developmental biology, confocal microscopy is widely used in plant cell imaging after the development of biological fluorescence labels in mid-1990s. However, fluorescence labeling itself affects the sample and the sample deviates from intact condition especially when labelling the entire cell. In this work, we report the dynamic images of Arabidopsis thaliana root cells. This demonstrates the multimodal nonlinear optical microscopy is an effective tool for long-term plant cell imaging.
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Bumjoon Jang, Bumjoon Jang, Sung-Ho Lee, Sung-Ho Lee, Sooah Woo, Sooah Woo, Jong-Hyun Park, Jong-Hyun Park, Myeong Min Lee, Myeong Min Lee, Seung-Han Park, Seung-Han Park, } "Multimodal nonlinear imaging of arabidopsis thaliana root cell", Proc. SPIE 10324, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2017, 103240C (12 July 2017); doi: 10.1117/12.2270697; https://doi.org/10.1117/12.2270697
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