RBCs as microlenses: wavefront analysis and applications

Francesco Merola; Álvaro Barroso; Lisa Miccio; Pasquale Memmolo; Martina Mugnano; Pietro Ferraro; Cornelia Denz

doi:10.1117/12.2272042

26 June 2017 RBCs as microlenses: wavefront analysis and applications

Francesco Merola, Álvaro Barroso, Lisa Miccio, Pasquale Memmolo, Martina Mugnano, Pietro Ferraro, Cornelia Denz

Proceedings Volume 10333, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials III; 1033311 (2017) https://doi.org/10.1117/12.2272042
Event: SPIE Optical Metrology, 2017, Munich, Germany

Abstract

Developing the recently discovered concept of RBCs as microlenses, we demonstrate further applications in wavefront analysis and diagnostics. Correlation between RBC’s morphology and its behavior as a refractive optical element has been established. In fact, any deviation from the healthy RBC morphology can be seen as additional aberration in the optical wavefront passing through the cell. By this concept, accurate localization of focal spots of RBCs can become very useful in blood disorders identification. Moreover, By modelling RBC as bio-lenses through Zernike polynomials it is possible to identify a series of orthogonal parameters able to recognise RBC shapes. The main improvement concerns the possibility to combine such parameters because of their independence conversely to standard image-based analysis where morphological factors are dependent each-others. We investigate the three-dimensional positioning of such focal spots over time for samples with two different osmolarity conditions, i.e. discocytes and spherocytes. Finally, Zernike polynomials wavefront analysis allows us to study the optical behavior of RBCs under an optically-induced mechanical stress. Detailed wavefront analysis provides comprehensive information about the aberrations induced by the deformation obtained using optical tweezers. This could open new routes for analyzing cell elasticity by examining optical parameters instead of direct but with low resolution strain analysis, thanks to the high sensitivity of the interferometric tool.

Citation Download Citation

Francesco Merola, Álvaro Barroso, Lisa Miccio, Pasquale Memmolo, Martina Mugnano, Pietro Ferraro, and Cornelia Denz "RBCs as microlenses: wavefront analysis and applications", Proc. SPIE 10333, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials III, 1033311 (26 June 2017); https://doi.org/10.1117/12.2272042

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KEYWORDS

Wavefront analysis

Microlens

Zernike polynomials

Blood

Diagnostics

Factor analysis

Image analysis

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