Optical methods for the evaluation of film-forming ability of UPEC on the surface of medical devices

Basim M. Al-Bayati; Olga V. Nechaeva; Vladimir Yu. Ulyanov; Elena V. Glinskaya; Dmitry A. Zayarsky; Elena I. Tikhomirova; Malika M. Vakaraeva; Natalia V. Bespalova; Anna V. Egorova

doi:10.1117/12.2267860

24 March 2017 Optical methods for the evaluation of film-forming ability of UPEC on the surface of medical devices

Basim M. Al-Bayati, Olga V. Nechaeva, Vladimir Yu. Ulyanov, Elena V. Glinskaya, Dmitry A. Zayarsky, Elena I. Tikhomirova, Malika M. Vakaraeva, Natalia V. Bespalova, Anna V. Egorova

Author Affiliations +

Proceedings Volume 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII; 103360E (2017) https://doi.org/10.1117/12.2267860
Event: Saratov Fall Meeting 2016: Fourth International Symposium on Optics and Biophotonics, 2016, Saratov, Russian Federation

Abstract

The current study was designed to investigate the dynamics of microbial biofilm-formation in standard and clinical strains of uropathogenic Escherichia coli (UPEC), using spectrophotometric and electron microscopic methods. For achieving this goal, three E. coli strains were used (one standard and two clinical). These isolates differ in the existence of fimH virulence gene. The process involved the using of electron microscopy and immunological microtitration plates under in vitro conditions for 96 hours. We found that standard and clinical strains of E. coli were capable of forming a microbial biofilm in vitro, with a higher intensity related to the clinical strain E. coli 245. The using of scanning electron microscopy confirmed the obtained results. The combined use of different optical methods gives a complete characterization of film-forming ability of pathogenic microorganisms.

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Basim M. Al-Bayati, Olga V. Nechaeva, Vladimir Yu. Ulyanov, Elena V. Glinskaya, Dmitry A. Zayarsky, Elena I. Tikhomirova, Malika M. Vakaraeva, Natalia V. Bespalova, and Anna V. Egorova "Optical methods for the evaluation of film-forming ability of UPEC on the surface of medical devices", Proc. SPIE 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII, 103360E (24 March 2017); https://doi.org/10.1117/12.2267860

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