Fluorescent angiography of chicken embryo and photobleaching velocimetry

Anton A. Namykin; Elena S. Stiukhina; Ivan V. Fedosov; Dmitry E. Postnov; Valery V. Tuchin

doi:10.1117/12.2267948

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24 March 2017 Fluorescent angiography of chicken embryo and photobleaching velocimetry

Anton A. Namykin, Elena S. Stiukhina, Ivan V. Fedosov, Dmitry E. Postnov, Valery V. Tuchin

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Proceedings Volume 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII; 103360V (2017) https://doi.org/10.1117/12.2267948
Event: Saratov Fall Meeting 2016: Fourth International Symposium on Optics and Biophotonics, 2016, Saratov, Russian Federation

Abstract

Fluorescent angiography approach in application to a living chicken embryo is discussed. It provides precise vessel wall detection and demonstrates usefulness for real time monitoring of vasoconstriction and vasodilatation related to self regulation of vascular network as well as to response to external factors. On the other hand, high stability of fluorescence and long period of dye elimination makes variations of fluorescent intensity practically independent from fast variations of blood flow rate. Therefore, we proposed the improvement of fluorescent angiography technique by introduction of photobleaching fluorescent velocimetry approach. We have developed the imaging system for intravital microscopic photobleaching velocimetry and tested it by using a glass capillary tube as a model of blood vessel. We demonstrated high potential of the technique for instant flow velocity distribution profile measurement with high spatial and temporal resolution up to 2 μm and 60 ms, respectively.

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Anton A. Namykin, Elena S. Stiukhina, Ivan V. Fedosov, Dmitry E. Postnov, and Valery V. Tuchin "Fluorescent angiography of chicken embryo and photobleaching velocimetry", Proc. SPIE 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII, 103360V (24 March 2017); https://doi.org/10.1117/12.2267948

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