24 March 2017 Laser speckle contrast imaging of cerebral blood flow of newborn mice at optical clearing
Author Affiliations +
Proceedings Volume 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII; 1033610 (2017) https://doi.org/10.1117/12.2268358
Event: Saratov Fall Meeting 2016: Fourth International Symposium on Optics and Biophotonics, 2016, Saratov, Russian Federation
Abstract
In this work, we consider the use of optical clearing agents to improve imaging quality of the cerebral blood flow of newborn mice. Aqueous 60%-glycerol solution, aqueous 70%-OmnipaqueTM(300) solution and OmnipaqueTM (300) solution in water/DMSO(25%/5%) were selected as the optical clearing agents. Laser speckle contrast imaging (LSCI) was used for imaging of cerebral blood flow in newborn mice brain during topical optical clearing of tissuesin the area of the fontanelle. These results demonstrate the effectiveness of glycerol and Omnipaque solutions as optical clearing agents for investigation of cerebral blood flow in newborn mice without scalp removing and skull thinning.
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Polina A. Timoshina, Ekaterina M. Zinchenko, Daria K. Tuchina, Madina M. Sagatova, Oxana V. Semyachkina-Glushkovskaya, Valery V. Tuchin, "Laser speckle contrast imaging of cerebral blood flow of newborn mice at optical clearing", Proc. SPIE 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII, 1033610 (24 March 2017); doi: 10.1117/12.2268358; https://doi.org/10.1117/12.2268358
PROCEEDINGS
7 PAGES


SHARE
Back to Top