Imaging galectin-3 dependent endocytosis with lattice light-sheet microscopy

Jongho Baek; Jieqiong Lou; Simao Coelho; Yean Jin Lim; Silvia Seidlitz; Philip R. Nicovich; Christian Wunder; Ludger Johannes; Katharina Gaus

doi:10.1117/12.2275706

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29 April 2017 Imaging galectin-3 dependent endocytosis with lattice light-sheet microscopy

Jongho Baek; Jieqiong Lou; Simao Coelho; Yean Jin Lim; Silvia Seidlitz; Philip R. Nicovich; Christian Wunder; Ludger Johannes; Katharina Gaus

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Proceedings Volume 10340, International Conference on Biophotonics V; 103400J (2017) https://doi.org/10.1117/12.2275706
Event: International Conference on Biophotonics V, 2017, Perth, Australia

Abstract

Lattice light-sheet (LLS) microscopy provides ultrathin light sheets of a two-dimensional optical lattice that allows us imaging three-dimensional (3D) objects for hundreds of time points at sub-second intervals and at or below the diffraction limit. Galectin-3 (Gal3), a carbohydrate-binding protein, triggers glycosphingolipid (GSL)-dependent biogenesis of morphologically distinct endocytic vesicles that are cargo specific and clathrin independent. In this study, we apply LLS microscopy to study the dynamics of Gal3 dependent endocytosis in live T cells. This will allow us to observe Gal3-mediated endocytosis at high temporal and excellent 3D spatial resolution, which may shed light on our understanding of the mechanism and physiological function of Gal3-induced endocytosis.

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Jongho Baek, Jieqiong Lou, Simao Coelho, Yean Jin Lim, Silvia Seidlitz, Philip R. Nicovich, Christian Wunder, Ludger Johannes, and Katharina Gaus "Imaging galectin-3 dependent endocytosis with lattice light-sheet microscopy", Proc. SPIE 10340, International Conference on Biophotonics V, 103400J (29 April 2017); https://doi.org/10.1117/12.2275706

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