29 April 2017 Functionalized gold nanostars for label-free detection of PKA phosphorylation using surface-enhanced Raman spectroscopy
Author Affiliations +
Proceedings Volume 10340, International Conference on Biophotonics V; 103400W (2017) https://doi.org/10.1117/12.2269722
Event: International Conference on Biophotonics V, 2017, Perth, Australia
Abstract
Protein phosphorylation controls fundamental biological processes. Dysregulation of protein kinase is associated with a series of human diseases including cancer. Protein kinase A (PKA) activity has been reported to serve as a potential prognostic marker for cancer. To this end, we developed a non-radioactive, rapid, cheap and robust scheme based on surface-enhanced Raman spectroscopy (SERS) for label-free detection of PKA phosphorylation using gold nanostars (AuNS) functionalized with BSA-kemptide. While bovine serum albumin (BSA) proteins stabilized the AuNS, kemptide, which is a high affinity substrate peptide specific for PKA, were phosphorylated in vitro to generate Raman signals that were identified by performing principal component analysis (PCA) on the acquired SERS spectra.
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Shuai He, Shuai He, James Chen Yong Kah, James Chen Yong Kah, } "Functionalized gold nanostars for label-free detection of PKA phosphorylation using surface-enhanced Raman spectroscopy", Proc. SPIE 10340, International Conference on Biophotonics V, 103400W (29 April 2017); doi: 10.1117/12.2269722; https://doi.org/10.1117/12.2269722
PROCEEDINGS
4 PAGES


SHARE
Back to Top