Ultrafast laser scanning cellular microscopy by spatiotemporally encoded virtual sources

Wenwei Yan; Jianglai Wu; Kenneth K. Y. Wong; Kevin K. Tsia

doi:10.1117/12.2270546

29 April 2017 Ultrafast laser scanning cellular microscopy by spatiotemporally encoded virtual sources

Wenwei Yan, Jianglai Wu, Kenneth K. Y. Wong, Kevin K. Tsia

Proceedings Volume 10340, International Conference on Biophotonics V; 103401A (2017) https://doi.org/10.1117/12.2270546
Event: International Conference on Biophotonics V, 2017, Perth, Australia

Abstract

We report a new type of all-optical ultrafast laser-scanning microscopy( (at a line-scan rate of 20 MHz) based on a phenomenon called free-space angular-chirp-enhanced delay (FACED). It results in the generation of a reconfigurable array of spatiotemporally encoded virtual pulsed sources, which acts as a scanning laser beam. We demonstrate its application in high-throughput multivariate image-based single-cell analysis (10,000 cells/sec).

Citation Download Citation

Wenwei Yan, Jianglai Wu, Kenneth K. Y. Wong, and Kevin K. Tsia "Ultrafast laser scanning cellular microscopy by spatiotemporally encoded virtual sources", Proc. SPIE 10340, International Conference on Biophotonics V, 103401A (29 April 2017); https://doi.org/10.1117/12.2270546

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