Handheld highly selective plasmonic chem/biosensor using engineered binding proteins for extreme conformational changes

Derek J. Kosciolek; Ajay Sonar; Lori A. Lepak; Peter Schnatz; Igor Bendoym; Mia C. Brown; Ronald L. Koder; David T. Crouse

doi:10.1117/12.2273207

25 August 2017 Handheld highly selective plasmonic chem/biosensor using engineered binding proteins for extreme conformational changes

Derek J. Kosciolek, Ajay Sonar, Lori A. Lepak, Peter Schnatz, Igor Bendoym, Mia C. Brown, Ronald L. Koder, David T. Crouse

Author Affiliations +

Proceedings Volume 10346, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XV; 103462B (2017) https://doi.org/10.1117/12.2273207
Event: SPIE Nanoscience + Engineering, 2017, San Diego, California, United States

Abstract

In this project we develop a handheld, portable, highly selective and sensitive chem/biosensor that has potential applications in both airborne and water-based environmental sensing. The device relies on a plasmonic chip of subwavelength-scale periodic gold rods engineered to resonate in the near infrared. The chip is functionalized with a novel class of proteins that exhibit large conformational changes upon binding to a specific target analyte. The subsequent change in local refractive index near the surface of the gold is one to two orders of magnitude greater than current conventional methods, which produces a readily measurable 5 to 10 percent difference in light transmission. This allows us to forgo traditional, bulky tabletop setups in favor of a compact form factor. Using commercially available optics to construct a transmission-based optical train, measured changes in bulk refractive index are presented here. While synthesis of binding protein efforts are focused on heme as analyte for proof of concept validation, the functionalized protein can be engineered to pair with a wide variety of analytes with minimal alterations to the plasmonic chip or device design. Such flexibility allows for this device to potentially meet the needs of first responders and health care professionals in a multitude of scenarios.

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Derek J. Kosciolek, Ajay Sonar, Lori A. Lepak, Peter Schnatz, Igor Bendoym, Mia C. Brown, Ronald L. Koder, and David T. Crouse "Handheld highly selective plasmonic chem/biosensor using engineered binding proteins for extreme conformational changes", Proc. SPIE 10346, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XV, 103462B (25 August 2017); https://doi.org/10.1117/12.2273207

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