Dielectrophoretic focusing integrated pulsed laser activated cell sorting

Xiongfeng Zhu; Yu-Chun Kung; Ting-Hsiang Wu; Michael A. Teitell; Pei-Yu Chiou

doi:10.1117/12.2275569

25 August 2017 Dielectrophoretic focusing integrated pulsed laser activated cell sorting

Xiongfeng Zhu, Yu-Chun Kung, Ting-Hsiang Wu, Michael A. Teitell, Pei-Yu Chiou

Proceedings Volume 10347, Optical Trapping and Optical Micromanipulation XIV; 103472K (2017) https://doi.org/10.1117/12.2275569
Event: SPIE Nanoscience + Engineering, 2017, San Diego, California, United States

Abstract

We present a pulsed laser activated cell sorter (PLACS) integrated with novel sheathless size-independent dielectrophoretic (DEP) focusing. Microfluidic fluorescence activated cell sorting (μFACS) systems aim to provide a fully enclosed environment for sterile cell sorting and integration with upstream and downstream microfluidic modules. Among them, PLACS has shown a great potential in achieving comparable performance to commercial aerosol-based FACS (>90% purity at 25,000 cells sec⁻¹). However conventional sheath flow focusing method suffers a severe sample dilution issue. Here we demonstrate a novel dielectrophoresis-integrated pulsed laser activated cell sorter (DEP-PLACS). It consists of a microfluidic channel with 3D electrodes laid out to provide a tunnel-shaped electric field profile along a 4cmlong channel for sheathlessly focusing microparticles/cells into a single stream in high-speed microfluidic flows. All focused particles pass through the fluorescence detection zone along the same streamline regardless of their sizes and types. Upon detection of target fluorescent particles, a nanosecond laser pulse is triggered and focused in a neighboring channel to generate a rapidly expanding cavitation bubble for precise sorting. DEP-PLACS has achieved a sorting purity of 91% for polystyrene beads at a throughput of 1,500 particle/sec.

Citation Download Citation

Xiongfeng Zhu, Yu-Chun Kung, Ting-Hsiang Wu, Michael A. Teitell, and Pei-Yu Chiou "Dielectrophoretic focusing integrated pulsed laser activated cell sorting", Proc. SPIE 10347, Optical Trapping and Optical Micromanipulation XIV, 103472K (25 August 2017); https://doi.org/10.1117/12.2275569

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