Non-degenerate two-photon excitation for increasing the fluorescence photon yield and maximum microscopy imaging depth (Conference Presentation)

Mu-Han Yang; Christopher G. L. Ferri; Payam A. Saisan; Maxim Abashin; Peifang Tian; Yeshayahu Fainman; Anna Devor

doi:10.1117/12.2274849

19 September 2017 Non-degenerate two-photon excitation for increasing the fluorescence photon yield and maximum microscopy imaging depth (Conference Presentation)

Mu-Han Yang, Christopher G. L. Ferri, Payam A. Saisan, Maxim Abashin, Peifang Tian, Yeshayahu Fainman, Anna Devor

Author Affiliations +

Proceedings Volume 10380, Ultrafast Nonlinear Imaging and Spectroscopy V; 103800Y (2017) https://doi.org/10.1117/12.2274849
Event: SPIE Optical Engineering + Applications, 2017, San Diego, California, United States

Abstract

We investigate the utility of non-degenerate 2-photon excitation (ND-2PE) as a strategy for extending the 2-photon imaging depth. For the ND-2PE scheme, two pulsed, synchronized laser sources of different wavelength each provide a photon for the 2-photon absorption process. By independently tuning their wavelengths, we are able to tune the excitation to tissue transparency windows while maintaining resonant fluorescence excitation. These transparency windows reduce excitation power loss resulting from scattering. In addition, by having two sources we are able to displace the beams in space except at their common focus; thus, reducing background fluorescence excitation. Finally, we show that ND-2PE inherently results in increased 2-photon absorption cross sections, resulting in increased fluorescence intensity. By combining beam displacement, tissue transparency and increased absorption cross sections, we achieve increased imaging depths as compared to degenerate 2-photon excitation with commonly used fluorophores.

Conference Presentation

Citation Download Citation

Mu-Han Yang, Christopher G. L. Ferri, Payam A. Saisan, Maxim Abashin, Peifang Tian, Yeshayahu Fainman, and Anna Devor "Non-degenerate two-photon excitation for increasing the fluorescence photon yield and maximum microscopy imaging depth (Conference Presentation)", Proc. SPIE 10380, Ultrafast Nonlinear Imaging and Spectroscopy V, 103800Y (19 September 2017); https://doi.org/10.1117/12.2274849

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available