27 September 2017 Imaging of the human tooth cementum ultrastructure of archeological teeth, using hard x-ray microtomography to determine age-at-death and stress periods
Author Affiliations +
Abstract
Tooth cementum annulation (TCA) is used by anthropologists to decipher age-at-death and stress periods based on yearly deposited incremental lines (ILs). The destructive aspect of the TCA method, which requires cutting the tooth root in sections to display the ILs, using transmission light microscopy, can be problematic for archeological teeth, and so a non-invasive imaging technique is preferred. The purpose of this study is to evaluate conventional micro computed tomography (μCT) and synchrotron radiation-based X-ray micro computed tomography (SRμCT) as a non-destructive technique to explore the tooth cementum ultrastructure and to display ILs. Seven archeological teeth from the Basel- Spitalfriedhof collection (patients died between 1845 and 1868 in the city hospital) were selected for the μCT experiments. This collection is considered a unique worldwide reference series in the anthropological science community, due to the high level of documented life history data in the medical files and the additionally collected and verified birth history by genealogists. The results demonstrate that the conventional μCT is complementary to the SRμCT allowing to prescreen the teeth using conventional μCT to identify the appropriate specimens and areas for the SRμCT measurements. SRμCT displayed cementum ring structure corresponding to the ILs in the microscope view in archeological teeth in a non-invasive fashion with the potential for more accurate assessments of ILs compared to conventional techniques. The ILs were mainly clearly visible, and it was possible to count them for age-at-death assessment and identify qualitatively irregular ILs which could constitute stress markers.
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Gabriela Mani-Caplazi, Gabriela Mani-Caplazi, Georg Schulz, Georg Schulz, Hans Deyhle, Hans Deyhle, Gerhard Hotz, Gerhard Hotz, Werner Vach, Werner Vach, Ursula Wittwer-Backofen, Ursula Wittwer-Backofen, Bert Müller, Bert Müller, } "Imaging of the human tooth cementum ultrastructure of archeological teeth, using hard x-ray microtomography to determine age-at-death and stress periods", Proc. SPIE 10391, Developments in X-Ray Tomography XI, 103911C (27 September 2017); doi: 10.1117/12.2276148; https://doi.org/10.1117/12.2276148
PROCEEDINGS
8 PAGES


SHARE
Back to Top