28 July 2017 Multimodal fiber-probe spectroscopy as a clinical tool for diagnosing and classifying biological tissues
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Abstract
An optical fiber probe for multimodal spectroscopy was designed, developed and used for tissue diagnostics. The probe, based on a fiber bundle with optical fibers of various size and properties, allows performing spectroscopic measurements with different techniques, including fluorescence, Raman, and diffuse reflectance, using the same probe. Two visible laser diodes were used for fluorescence spectroscopy, a laser diode emitting in the NIR was used for Raman spectroscopy, and a fiber-coupled halogen lamp for diffuse reflectance. The developed probe was successfully employed for diagnostic purposes on various tissues, including brain and bladder. In particular, the device allowed discriminating healthy tissue from both tumor and dysplastic tissue as well as to perform tumor grading. The diagnostic capabilities of the method, determined using a cross-validation method with a leave-one-out approach, demonstrated high sensitivity and specificity for all the examined samples, as well as a good agreement with histopathological examination performed on the same samples. The obtained results demonstrated that the multimodal approach is crucial for improving diagnostic capabilities with respect to what can be obtained from individual techniques. The experimental setup presented here can improve diagnostic capabilities on a broad range of tissues and has the potential of being used clinically for guiding surgical resection in the near future.
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Riccardo Cicchi, Riccardo Cicchi, Suresh Anand, Suresh Anand, Riccardo Fantechi, Riccardo Fantechi, Flavio Giordano, Flavio Giordano, Mauro Gacci, Mauro Gacci, Valerio Conti, Valerio Conti, Gabriella Nesi, Gabriella Nesi, Anna Maria Buccoliero, Anna Maria Buccoliero, Marco Carini, Marco Carini, Renzo Guerrini, Renzo Guerrini, Francesco Saverio Pavone, Francesco Saverio Pavone, } "Multimodal fiber-probe spectroscopy as a clinical tool for diagnosing and classifying biological tissues", Proc. SPIE 10411, Clinical and Preclinical Optical Diagnostics, 104110E (28 July 2017); doi: 10.1117/12.2283278; https://doi.org/10.1117/12.2283278
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