There is a pressing clinical need to provide image guidance during surgery. Currently, assessment of tissue that needs to
be resected or avoided is performed subjectively leading to a large number of failures, patient morbidity and increased
healthcare cost. Because near-infrared (NIR) optical imaging is safe, does not require contact, and can provide relatively
deep information (several mm), it offers unparalleled capabilities for providing image guidance during surgery. In this
work, we introduce a novel concept that enables the quantitative imaging of endogenous molecular information over
large fields-of-view. Because this concept can be implemented in real-time, it is amenable to provide video-rate
endogenous information during surgery.