28 July 2017 Multi-spectral endogenous fluorescence imaging for bacterial differentiation
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Abstract
In this paper, the multi-spectral endogenous fluorescence imaging was implemented for bacterial differentiation. The fluorescence imaging was performed using a digital camera equipped with a set of visual bandpass filters. Narrowband 365 nm ultraviolet radiation passed through a beam homogenizer was used to excite the sample fluorescence. In order to increase a signal-to-noise ratio and suppress a non-fluorescence background in images, the intensity of the UV excitation was modulated using a mechanical chopper. The principal components were introduced for differentiating the samples of bacteria based on the multi-spectral endogenous fluorescence images.
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Nikita V. Chernomyrdin, Margarita V. Babayants, Oleg V. Korotkov, Konstantin G. Kudrin, Elena N. Rimskaya, Irina A. Shikunova, Vladimir N. Kurlov, Olga P. Cherkasova, Gennady A. Komandin, Igor V. Reshetov, Kirill I. Zaytsev, "Multi-spectral endogenous fluorescence imaging for bacterial differentiation", Proc. SPIE 10411, Clinical and Preclinical Optical Diagnostics, 104110B (28 July 2017); doi: 10.1117/12.2285065; http://dx.doi.org/10.1117/12.2285065
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Luminescence

Auto-fluorescence imaging

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