28 July 2017 Improvements in low-cost label-free QPI microscope for live cell imaging
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Abstract
This paper reports an improvement in the development of a low-cost QPI microscope offering new capabilities in term of phase measurement accuracy for label-free live samples in the longer term (i.e., hours to days). The spatially separated scattered and non-scattered image light fields are reshaped in the Fourier plane and modulated to form an interference image at a CCD camera. The apertures that enable these two beams to be generated have been optimised by means of laser-cut apertures placed on the mirrors of a Michelson interferometer and has improved the phase measuring and reconstruction capability of the QPI microscope. The microscope was tested with transparent onion cells as an object of interest.
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C. Seniya, C. Seniya, C. E. Towers, C. E. Towers, D. P. Towers, D. P. Towers, } "Improvements in low-cost label-free QPI microscope for live cell imaging", Proc. SPIE 10414, Advances in Microscopic Imaging, 104140F (28 July 2017); doi: 10.1117/12.2282230; https://doi.org/10.1117/12.2282230
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