Optimal staining and clearing protocol for whole mouse brain vasculature imaging with light-sheet microscopy

Antonino Paolo Di Giovanna; Alessandro Tibo; Ludovico Silvestri; Marie Caroline Müllenbroich; Irene Costantini; Leonardo Sacconi; Paolo Frasconi; Francesco Saverio Pavone

doi:10.1117/12.2285044

28 July 2017 Optimal staining and clearing protocol for whole mouse brain vasculature imaging with light-sheet microscopy

Antonino Paolo Di Giovanna, Alessandro Tibo, Ludovico Silvestri, Marie Caroline Müllenbroich, Irene Costantini, Leonardo Sacconi, Paolo Frasconi, Francesco Saverio Pavone

Author Affiliations +

Proceedings Volume 10414, Advances in Microscopic Imaging; 104140N (2017) https://doi.org/10.1117/12.2285044
Event: European Conferences on Biomedical Optics, 2017, Munich, Germany

Abstract

Light-sheet microscopy enables whole mouse brain imaging in association with clearing methodologies. Here, we present a pipeline for optimal investigation of the vascular component, which offers improved image quality for morphological analysis.

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Antonino Paolo Di Giovanna, Alessandro Tibo, Ludovico Silvestri, Marie Caroline Müllenbroich, Irene Costantini, Leonardo Sacconi, Paolo Frasconi, and Francesco Saverio Pavone "Optimal staining and clearing protocol for whole mouse brain vasculature imaging with light-sheet microscopy", Proc. SPIE 10414, Advances in Microscopic Imaging, 104140N (28 July 2017); https://doi.org/10.1117/12.2285044

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