13 June 2017 Multimodal quantitative phase and fluorescence imaging of cell apoptosis
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Proceedings Volume 10449, Fifth International Conference on Optical and Photonics Engineering; 1044920 (2017) https://doi.org/10.1117/12.2270794
Event: Fifth International Conference on Optical and Photonics Engineering, 2017, Singapore, Singapore
Abstract
Fluorescence microscopy, utilizing fluorescence labeling, has the capability to observe intercellular changes which transmitted and reflected light microscopy techniques cannot resolve. However, the parts without fluorescence labeling are not imaged. Hence, the processes simultaneously happen in these parts cannot be revealed. Meanwhile, fluorescence imaging is 2D imaging where information in the depth is missing. Therefore the information in labeling parts is also not complete.

On the other hand, quantitative phase imaging is capable to image cells in 3D in real time through phase calculation. However, its resolution is limited by the optical diffraction and cannot observe intercellular changes below 200 nanometers.

In this work, fluorescence imaging and quantitative phase imaging are combined to build a multimodal imaging system. Such system has the capability to simultaneously observe the detailed intercellular phenomenon and 3D cell morphology. In this study the proposed multimodal imaging system is used to observe the cell behavior in the cell apoptosis. The aim is to highlight the limitations of fluorescence microscopy and to point out the advantages of multimodal quantitative phase and fluorescence imaging. The proposed multimodal quantitative phase imaging could be further applied in cell related biomedical research, such as tumor.
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Xinye Fu, Xinye Fu, Chao Zuo, Chao Zuo, Hao Yan, Hao Yan, } "Multimodal quantitative phase and fluorescence imaging of cell apoptosis", Proc. SPIE 10449, Fifth International Conference on Optical and Photonics Engineering, 1044920 (13 June 2017); doi: 10.1117/12.2270794; https://doi.org/10.1117/12.2270794
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