Methodology of mycobacteria tuberculosis bacteria detection by Raman spectroscopy

A. Zyubin; A. Lavrova; O. Manicheva; M. Dogonadze; A. Tsibulnikova; I. Samusev

doi:10.1117/12.2282967

2 January 2018 Methodology of mycobacteria tuberculosis bacteria detection by Raman spectroscopy

A. Zyubin, A. Lavrova, O. Manicheva, M. Dogonadze, A. Tsibulnikova, I. Samusev

Proceedings Volume 10456, Nanophotonics Australasia 2017; 104565L (2018) https://doi.org/10.1117/12.2282967
Event: SPIE Nanophotonics Australasia, 2017, Melbourne, Australia

Abstract

We have developed a methodology for the study of deactivated strains of Mycobacterium tuberculosis. Strains of the Beijing species obtained from pulmonary patient secrete (XDR strain) and reference strain (H37Rv) were investigated by Raman spectrometry with He-Ne (632,8 nm) laser excitation source. As a result of the research, the optimal experimental parameters have been obtained to get spectra of mycolic acids, which are part of the cell wall of mycobacteria.

Citation Download Citation

A. Zyubin, A. Lavrova, O. Manicheva, M. Dogonadze, A. Tsibulnikova, and I. Samusev "Methodology of mycobacteria tuberculosis bacteria detection by Raman spectroscopy", Proc. SPIE 10456, Nanophotonics Australasia 2017, 104565L (2 January 2018); https://doi.org/10.1117/12.2282967

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