With much more attention and utilizing paying to space resource, the detection and removal of space debris, the biggest threatened to the orbiting spacecraft, has become a research hotspot in recent years. In order to protect the important space assets, such as the international space station, it has been realized of simulation and system parameters’ design, which contained debris’ detection and removal by space-based pulse laser. Simulation results show that the determine time of detection laser and removal laser should be considered after judging weather debris is in the “clear window”. As the increasing of detection pulse and removal pulse, the orbit element of space debris has a regular change, with the decreasing of single pulse velocity increment. The system of detection and removal of space debris designed as: detection laser power 50W, removal laser power 150kW, laser wavelength 1064nm, pulse width 10ns, frequency 100Hz. The research has a great significance of detection and removal of debris by space-based laser and engineering application.