24 October 2017 Subcellular analysis of interaction between breast cancer cells and drug by digital holography
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Proceedings Volume 10458, AOPC 2017: 3D Measurement Technology for Intelligent Manufacturing; 104581B (2017) https://doi.org/10.1117/12.2285271
Event: Applied Optics and Photonics China (AOPC2017), 2017, Beijing, China
Abstract
Digital holographic microscopy is a promising quantitative phase-contrast imaging technique, which exhibits the advantages of non-destruction, full field of view, quasi-real time, and don’t need dye and external marker to the living biological sample. In this paper, the inverted off-axis image-plane digital holography with pre-magnification is built up to study the living MDA-MB-231 breast cancer cells. The lateral resolution of the proposed experimental setup is 0.87μm, which is verified by the standard USAF test target. Then the system is used to visualize the interaction between living breast cancer cells and drug. The blebbing is observed after the cells are treated by paclitaxel drug, and the distribution of the paclitaxel inside the cells is detected, which is near the cytomembrane, or in other words the end of the microtubules. It will stop the mitosis and cause the death of the cells. It is helpful to reveal the anticancer mechanism of paclitaxel in the subcellular scale.
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Jie Zhao, Jie Zhao, Qiaowen Lin, Qiaowen Lin, Dayong Wang, Dayong Wang, Yunxin Wang, Yunxin Wang, Liting Ouyang, Liting Ouyang, Sha Guo, Sha Guo, Qian Yao, Qian Yao, } "Subcellular analysis of interaction between breast cancer cells and drug by digital holography", Proc. SPIE 10458, AOPC 2017: 3D Measurement Technology for Intelligent Manufacturing, 104581B (24 October 2017); doi: 10.1117/12.2285271; https://doi.org/10.1117/12.2285271
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