Adaptive optics with combined optical coherence tomography and scanning laser ophthalmoscopy for in vivo mouse retina imaging

Pengfei Zhang; Daniel J. Wahl; Jacopo Mocci; Suman Manna; Ratheesh K. Meleppat; Stefano Bonora; Marinko V. Sarunic; Edward N. Pugh Jr.; Robert J. Zawadzki

doi:10.1117/12.2288607

18 March 2018 Adaptive optics with combined optical coherence tomography and scanning laser ophthalmoscopy for in vivo mouse retina imaging

Pengfei Zhang, Daniel J. Wahl, Jacopo Mocci, Suman Manna, Ratheesh K. Meleppat, Stefano Bonora, Marinko V. Sarunic, Edward N. Pugh Jr., Robert J. Zawadzki

Author Affiliations +

Proceedings Volume 10474, Ophthalmic Technologies XXVIII; 1047427 (2018) https://doi.org/10.1117/12.2288607
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Optical coherence tomography (OCT) and scanning laser ophthalmoscopy (SLO) are two state-of-the-art imaging technologies commonly used to study retina. Adaptive Optics (AO) methodologies enable high-fidelity correction of ocular aberrations, resulting in improved resolution and sensitivity for both SLO and OCT systems. Here we present work integrating OCT into a previously described mouse retinal AO-SLO system, allowing simultaneous reflectance and fluorescence imaging. The new system allows simultaneous data acquisition of AO-SLO and AO-OCT, facilitating registration and comparison of data from both modalities. The system has data acquisition speed of 200 kHz A-scans/pixel, and high volumetric resolution.

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Pengfei Zhang, Daniel J. Wahl, Jacopo Mocci, Suman Manna, Ratheesh K. Meleppat, Stefano Bonora, Marinko V. Sarunic, Edward N. Pugh Jr., and Robert J. Zawadzki "Adaptive optics with combined optical coherence tomography and scanning laser ophthalmoscopy for in vivo mouse retina imaging", Proc. SPIE 10474, Ophthalmic Technologies XXVIII, 1047427 (18 March 2018); https://doi.org/10.1117/12.2288607

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