CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 10475 > Article
Paper
7 February 2018 Fluorescence assessment of the delivery and distribution of nebulized indocyanine green in a murine model
Giulia Kassab, Mariana C. Geralde, Natalia M. Inada, Vanderlei S. Bagnato
Author Affiliations +
Proceedings Volume 10475, Visualizing and Quantifying Drug Distribution in Tissue II; 104750M (2018) https://doi.org/10.1117/12.2293013
Event: SPIE BiOS, 2018, San Francisco, California, United States
Abstract
Photodynamic inactivation (PDI) is a promising alternative for the treatment of infectious diseases, and the combination of indocyanine green (ICG) and extracorporeal infrared light has shown optimistic results against pneumonia in vitro and in vivo. However, the pharmacokinetics and the possible side effects of the pulmonary delivery via nebulization have not been fully investigated. This study assessed the distribution of the photosensitizer within the lungs and to other organs of mice, and monitored the fluorescence of ICG in the thorax in the presence and absence of the activating light. The excitation wavelength was 780 nm and detection focused on the emission between 795 and 890 nm. Experiments demonstrated that the amount of fluorescence detected from outside the body was significantly higher after the nebulization of ICG, and reduced after the illumination, allowing for the monitoring of the PDI in real time. The fluorescence remained detectable in the mice for at least 24 hours, and was present in the lungs, stomach, liver, small and large intestines, bladder, spleen and heart after this time.
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Giulia Kassab, Mariana C. Geralde, Natalia M. Inada, and Vanderlei S. Bagnato "Fluorescence assessment of the delivery and distribution of nebulized indocyanine green in a murine model", Proc. SPIE 10475, Visualizing and Quantifying Drug Distribution in Tissue II, 104750M (7 February 2018); https://doi.org/10.1117/12.2293013
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 7 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Photodynamic therapy
Fluorescence spectroscopy
Liver
Lung
Indocyanine green
Stomach
Infrared radiation
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top