8 February 2018 Blue light-irradiated human keloid fibroblasts: an in vitro study
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Abstract
Blue LED light irradiation is currently under investigation because of its effect in wound healing improvement. In this context, several mechanisms of action are likely to occur at the same time, consistently with the presence of different light absorbers within the skin. In our previous studies we observed the wound healing in superficial abrasions in an in vivo murine model. The results evidenced that both inflammatory infiltrate and myofibroblasts activity increase after irradiation. In this study we focused on evaluating the consequences of light absorption in fibroblasts from human cells culture: they play a key role in wound healing, both in physiological conditions and in pathological ones, such as keloid scarring. In particular we used keloids fibroblasts as a new target in order to investigate a possible metabolic or cellular mechanism correlation. Human keloid tissues were excised during standard surgery and immediately underwent primary cell culture extraction. Fibroblasts were allowed to grow in the appropriate conditions and then exposed to blue light. A metabolic colorimetric test (WST-8) was then performed. The tests evidenced an effect in mitochondrial activity, which could be modulated by the duration of the treatment. Electrophysiology pointed out a different behavior of irradiated fibroblasts. In conclusion, the Blue LED light affects the metabolic activity of fibroblasts and thus the cellular proliferation rate. No specific effect was found on keloid fibroblasts, thus indicating a very basic intracellular component, such as cytochromes, being the target of the treatment.
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Giada Magni, Francesca Rossi, Francesca Tatini, Roberto Pini, Elisabetta Coppi, Federica Cherchi, Irene Fusco, Anna Maria Pugliese, Felicita Pedata, Marco Fraccalvieri, Stefano Gasperini, Francesco S. Pavone, Cristina Tripodi, Domenico Alfieri, Lorenzo Targetti, "Blue light-irradiated human keloid fibroblasts: an in vitro study", Proc. SPIE 10477, Mechanisms of Photobiomodulation Therapy XIII, 104770A (8 February 2018); doi: 10.1117/12.2289928; https://doi.org/10.1117/12.2289928
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