8 February 2018 Staphyloxanthin photobleaching sensitizes methicillin-resistant Staphylococcus aureus to reactive oxygen species attack
Author Affiliations +
Given that the dearth of new antibiotic development loads an existential burden on successful infectious disease therapy, health organizations are calling for alternative approaches to combat methicillin-resistant Staphylococcus aureus (MRSA) infections. Here, we report a drug-free photonic approach to eliminate MRSA through photobleaching of staphyloxanthin, an indispensable membrane-bound antioxidant of S. aureus. The photobleaching process, uncovered through a transient absorption imaging study and quantitated by absorption spectroscopy and mass spectrometry, decomposes staphyloxanthin, and sensitizes MRSA to reactive oxygen species attack. Consequently, staphyloxanthin bleaching by low-level blue light eradicates MRSA synergistically with external or internal reactive oxygen species. The effectiveness of this synergistic therapy is validated in MRSA culture, MRSAinfected macrophage cells. Collectively, these findings highlight broad applications of staphyloxanthin photobleaching for treatment of MRSA infections.
Conference Presentation
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Pu-Ting Dong, Pu-Ting Dong, Haroon Mohammad, Haroon Mohammad, Jie Hui, Jie Hui, Xiaoyu Wang, Xiaoyu Wang, Junjie Li, Junjie Li, Lijia Liang, Lijia Liang, Mohamed N. Seleem, Mohamed N. Seleem, Ji-Xin Cheng, Ji-Xin Cheng, } "Staphyloxanthin photobleaching sensitizes methicillin-resistant Staphylococcus aureus to reactive oxygen species attack", Proc. SPIE 10479, Light-Based Diagnosis and Treatment of Infectious Diseases, 104790R (8 February 2018); doi: 10.1117/12.2291248; https://doi.org/10.1117/12.2291248

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