8 February 2018 Photodynamic therapy to destroy pneumonia associated microorganisms using external irradiation source
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Abstract
An endotracheal tube (ETT) is required for the management of critically ill, mechanically ventilated patients. Ventilatorassociated pneumonia (VAP) affects patients hospitalized in intensive care units; its risk of occurrence is 1% to up 3% for each day of mechanical ventilation. The polymicrobial nature of VAP is established with mixed bacterial-fungal biofilms colonizing the ETT. The microbial interaction enhances the microbial pathogenesis contributing to high indexes of morbidity/mortality. Antimicrobial Photodynamic Therapy (aPDT) could be a suitable therapy for decontamination of oral cavity and ETT at the same time, but the use of a fiber optics inside the ETT seems to not be appropriated since a cannula for secretion aspiration has to be introduced into the ETT to keep it´s lumen. The aim of this study is to proof the concept that an external light source from a LED is capable of reach all areas of the ETT. We use a commercial ETT, 60μM methylene blue (MB), and a 660nm diode laser and calculated the transmission coefficient of light in different situations as only tube, tube with biofilm and biofilm+MB. The results prove that is possible to transmit light through the tube even in the presence of MB and biofilm although a high attenuation of about 60% was measured depending on the tested condition.
Conference Presentation
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Rosane Bassi, Walter Myakawa, Ricardo S. Navarro, Alessandra Baptista, Martha Simões Ribeiro, Silvia Cristina Nunez, "Photodynamic therapy to destroy pneumonia associated microorganisms using external irradiation source", Proc. SPIE 10479, Light-Based Diagnosis and Treatment of Infectious Diseases, 1047917 (8 February 2018); doi: 10.1117/12.2290764; https://doi.org/10.1117/12.2290764
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