8 February 2018 Use of a conformational switching aptamer for rapid and specific ex vivo identification of central nervous system lymphoma in a xenograft model
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Abstract
Improved tools for providing specific intraoperative diagnoses could improve patient care. In neurosurgery, intraoperatively differentiating non-operative lesions can be challenging, often necessitating immunohistochemical (IHC) procedures which require up to 24-48 hours. Here, we evaluate the feasibility of generating rapid ex vivo specific labeling using a novel lymphoma-specific fluorescent switchable aptamer. Our B-cell lymphoma-specific switchable aptamer produced only low-level fluorescence in its unbound conformation and generated an 8-fold increase in fluorescence once bound to its target on CD20-positive lymphoma cells. The aptamer demonstrated strong binding to B-cell lymphoma cells within 10 minutes of incubation. We applied the switchable aptamer to ex vivo xenograft tissue harboring B-cell lymphoma and astrocytoma, and within one hour specific visual identification of lymphoma was routinely possible. In this proof-of-concept study in human cell culture and orthotopic xenografts, we conclude that a fluorescent switchable aptamer can provide rapid and specific labeling of B-cell lymphoma, and that developing aptamer-based labeling approaches could simplify tissue staining and drastically reduce time to histopathological diagnoses compared with IHC-based methods. We propose that switchable aptamers could enhance expeditious, accurate intraoperative decision-making.
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Joseph F. Georges, Joseph F. Georges, Xiaowei Liu, Xiaowei Liu, Jennifer Eschbacher, Jennifer Eschbacher, Joshua Nichols, Joshua Nichols, Michael A. Mooney, Michael A. Mooney, Anna Joy, Anna Joy, Robert F. Spetzler, Robert F. Spetzler, Burt G. Feuerstein , Burt G. Feuerstein , Trent Anderson, Trent Anderson, Mark C. Preul, Mark C. Preul, Hao Yan, Hao Yan, Peter Nakaji, Peter Nakaji, } "Use of a conformational switching aptamer for rapid and specific ex vivo identification of central nervous system lymphoma in a xenograft model", Proc. SPIE 10480, Clinical and Translational Neurophotonics 2018, 104800G (8 February 2018); doi: 10.1117/12.2290883; https://doi.org/10.1117/12.2290883
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