12 February 2018 UbasM: a simple, rapid, efficient balanced optical clearing method for brain imaging
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Proceedings Volume 10481, Neural Imaging and Sensing 2018; 104810W (2018) https://doi.org/10.1117/12.2283223
Event: SPIE BiOS, 2018, San Francisco, California, United States
Abstract
Optical clearing methods are highly in demand in organism-level biomedical system research since they can facilitate deep optical imaging by reducing light scattering in tissue and then enable three-dimensional signal visualization and quantification of tissues. While the previously reported optical clearing methods have addressed some of six key issues (i.e. transparency, efficiency, reproducibility, preservation of emission from fluorescence proteins, preservation of membrane integrity, and the ease of operation), none has yet addressed all of them. Here, we present a new, convenient, inexpensive and reproducible approach to optical clearing, termed UbasM, providing unprecedented performance in terms of clearing rate, the ease of operation and satisfactory fluorescence protein/membrane integrity preservation while achieving sufficient transparency to permit 3D volumetric imaging.
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Lingling Chen, Lingling Chen, Guiye Li, Guiye Li, Yingchao Li, Yingchao Li, Lina Liu, Lina Liu, Ang Liu, Ang Liu, Shuangchen Ruan, Shuangchen Ruan, } "UbasM: a simple, rapid, efficient balanced optical clearing method for brain imaging ", Proc. SPIE 10481, Neural Imaging and Sensing 2018, 104810W (12 February 2018); doi: 10.1117/12.2283223; https://doi.org/10.1117/12.2283223
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