12 February 2018 High-throughput isotropic mapping of whole mouse brain using multi-view light-sheet microscopy
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Proceedings Volume 10481, Neural Imaging and Sensing 2018; 104811X (2018) https://doi.org/10.1117/12.2295273
Event: SPIE BiOS, 2018, San Francisco, California, United States
Abstract
Light-sheet fluorescence microscopy (LSFM) uses an additional laser-sheet to illuminate selective planes of the sample, thereby enabling three-dimensional imaging at high spatial-temporal resolution. These advantages make LSFM a promising tool for high-quality brain visualization. However, even by the use of LSFM, the spatial resolution remains insufficient to resolve the neural structures across a mesoscale whole mouse brain in three dimensions. At the same time, the thick-tissue scattering prevents a clear observation from the deep of brain. Here we use multi-view LSFM strategy to solve this challenge, surpassing the resolution limit of standard light-sheet microscope under a large field-of-view (FOV). As demonstrated by the imaging of optically-cleared mouse brain labelled with thy1-GFP, we achieve a brain-wide, isotropic cellular resolution of ~3μm. Besides the resolution enhancement, multi-view braining imaging can also recover complete signals from deep tissue scattering and attenuation. The identification of long distance neural projections across encephalic regions can be identified and annotated as a result.
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Jun Nie, Jun Nie, Yusha Li, Yusha Li, Fang Zhao, Fang Zhao, Junyu Ping, Junyu Ping, Sa Liu, Sa Liu, Tingting Yu, Tingting Yu, Dan Zhu, Dan Zhu, Peng Fei, Peng Fei, } "High-throughput isotropic mapping of whole mouse brain using multi-view light-sheet microscopy", Proc. SPIE 10481, Neural Imaging and Sensing 2018, 104811X (12 February 2018); doi: 10.1117/12.2295273; https://doi.org/10.1117/12.2295273
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