An all-optical system for rapid and deep interrogation of behaviorally relevant activity patterns (Conference Presentation)

Gilad Lerman; Jonathan V. Gill; Dmitry Rinberg; Shy Shoham

doi:10.1117/12.2290850

14 March 2018 An all-optical system for rapid and deep interrogation of behaviorally relevant activity patterns (Conference Presentation)

Gilad Lerman, Jonathan V. Gill, Dmitry Rinberg, Shy Shoham

Author Affiliations +

Proceedings Volume 10482, Optogenetics and Optical Manipulation 2018; 104820U (2018) https://doi.org/10.1117/12.2290850
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Holographic optogenetics is an emerging tool for distributed control of spatiotemporal neuronal activity. Establishing causality between specific sequences of neuronal activity and behavior requires manipulating this code at the level of individual neurons while recording neural responses and behavioral readout. However, traditional methods of optogenetic perturbation lack the ability to emulate natural patterns of neural activity, or to rapidly alter the activity of specific neurons deep in the brain. To address the goal of producing behaviorally relevant sequences of neural activity, we have developed an all-optical, rapid two-photon optogenetic stimulation and imaging system with cellular resolution and 5 ms temporal precision. Using an amplified laser with high peak pulse power, together with wavefront shaping methods using a fast spatial light modulator (3 ms switching time), we were able to stimulate dozens of neurons deep in the olfactory bulb (>350 µm) at a high rate (>100 Hz) with cellular resolution. We optimized the system parameters to enable efficient excitation with a low power budget, to enable the simultaneous stimulation of many cells (~60). We then demonstrated stimulation of mitral and tufted cells, the projection neurons of the olfactory bulb, at a high rate, generating artificial odor-evoked responses. We will present the system characteristics and discuss its potential applications for manipulating and reading neuronal activity on a behaviorally relevant spatiotemporal scale to dissect the activity codes that guide behavior across different modalities.

Conference Presentation

Citation Download Citation

Gilad Lerman, Jonathan V. Gill, Dmitry Rinberg, and Shy Shoham "An all-optical system for rapid and deep interrogation of behaviorally relevant activity patterns (Conference Presentation)", Proc. SPIE 10482, Optogenetics and Optical Manipulation 2018, 104820U (14 March 2018); https://doi.org/10.1117/12.2290850

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Neurons

Optogenetics

Holography

Brain

Image resolution

Imaging systems

Pulsed laser operation

Show All Keywords

Keywords/Phrases

Search In:

Publication Years