14 February 2018 Optical coherence tomography of lymphatic vessel endothelial hyaluronan receptors in vivo
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Abstract
Optical Coherence Tomography (OCT) imaging of living subjects offers millimeters depth of penetration into tissue while maintaining high spatial resolution. However, because most molecular biomarkers do not produce inherent OCT contrast signals, exogenous contrast agents must be employed to achieve molecular imaging. Here we demonstrate that microbeads (μBs) can be used as effective contrast agents to target cellular biomarkers in lymphatic vessels and can be detected by OCT using a phase variance algorithm. We applied this technique to image the molecular dynamics of lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) in vivo, which showed significant down-regulation during tissue inflammation.
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Peng Si, Debasish Sen, Rebecca Dutta, Siavash Yousefi, Roopa Dalal, Yonatan Winetraub, Orly Liba, Adam de la Zerda, "Optical coherence tomography of lymphatic vessel endothelial hyaluronan receptors in vivo", Proc. SPIE 10483, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXII, 1048326 (14 February 2018); doi: 10.1117/12.2282890; https://doi.org/10.1117/12.2282890
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