14 February 2018 Optical coherence tomography of lymphatic vessel endothelial hyaluronan receptors in vivo
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Optical Coherence Tomography (OCT) imaging of living subjects offers millimeters depth of penetration into tissue while maintaining high spatial resolution. However, because most molecular biomarkers do not produce inherent OCT contrast signals, exogenous contrast agents must be employed to achieve molecular imaging. Here we demonstrate that microbeads (μBs) can be used as effective contrast agents to target cellular biomarkers in lymphatic vessels and can be detected by OCT using a phase variance algorithm. We applied this technique to image the molecular dynamics of lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) in vivo, which showed significant down-regulation during tissue inflammation.
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Peng Si, Peng Si, Debasish Sen, Debasish Sen, Rebecca Dutta, Rebecca Dutta, Siavash Yousefi, Siavash Yousefi, Roopa Dalal, Roopa Dalal, Yonatan Winetraub, Yonatan Winetraub, Orly Liba, Orly Liba, Adam de la Zerda, Adam de la Zerda, } "Optical coherence tomography of lymphatic vessel endothelial hyaluronan receptors in vivo", Proc. SPIE 10483, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXII, 1048326 (14 February 2018); doi: 10.1117/12.2282890; https://doi.org/10.1117/12.2282890

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