14 February 2018 Capillary red blood cell velocimetry by phase-resolved optical coherence tomography
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Abstract
Quantitative measurement of blood flow velocity in capillaries is challenging due to their small size (around 5-10 μm), and the discontinuity and single-file feature of RBCs flowing in a capillary. In this work, we present a phase-resolved Optical Coherence Tomography (OCT) method for accurate measurement of the red blood cell (RBC) speed in cerebral capillaries. To account for the discontinuity of RBCs flowing in capillaries, we applied an M-mode scanning strategy that repeated A-scans at each scanning position for an extended time. As the capillary size is comparable to the OCT resolution size (3.5×3.5×3.5μm), we applied a high pass filter to remove the stationary signal component so that the phase information of the dynamic component (i.e. from the moving RBC) could be enhanced to provide an accurate estimate of the RBC axial speed. The phase-resolved OCT method accurately quantifies the axial velocity of RBC’s from the phase shift of the dynamic component of the signal. We validated our measurements by RBC passage velocimetry using the signal magnitude of the same OCT time series data. These proposed method of capillary velocimetry proved to be a robust method of mapping capillary RBC speeds across the micro-vascular network.
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Jianbo Tang, Jianbo Tang, Sefik Evren Erdener, Sefik Evren Erdener, Buyin Fu, Buyin Fu, David A. Boas, David A. Boas, } "Capillary red blood cell velocimetry by phase-resolved optical coherence tomography ", Proc. SPIE 10483, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXII, 104832W (14 February 2018); doi: 10.1117/12.2290655; https://doi.org/10.1117/12.2290655
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