13 February 2018 Frequency-domain photoacoustic and fluorescence microscopy: application on labeled and unlabeled cells
Author Affiliations +
Proceedings Volume 10487, Multimodal Biomedical Imaging XIII; 1048709 (2018) https://doi.org/10.1117/12.2290023
Event: SPIE BiOS, 2018, San Francisco, California, United States
In this paper, multimodal optical-resolution frequency-domain photoacoustic and fluorescence scanning microscopy is presented on labeled and unlabeled cells. In many molecules, excited electrons relax radiatively and non-radiatively, leading to fluorescence and photoacoustic signals, respectively. Both signals can then be detected simultaneously. There also exist molecules, e.g. hemoglobin, which do not exhibit fluorescence, but provide photoacoustic signals solely. Other molecules, especially fluorescent dyes, preferentially exhibit fluorescence. The fluorescence quantum yield of a molecule and with it the strength of photoacoustic and fluorescence signals depends on the local environment, e.g. on the pH. Therefore, the local distribution of the simultaneously recorded photoacoustic and fluorescence signals may be used in order to obtain information about the local chemistry.
Conference Presentation
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Gregor Langer, Gregor Langer, Bianca Buchegger, Bianca Buchegger, Jaroslaw Jacak, Jaroslaw Jacak, Karoline Pfeffer, Karoline Pfeffer, Sven Wohlfarth, Sven Wohlfarth, Günther Hannesschläger, Günther Hannesschläger, Thomas A. Klar, Thomas A. Klar, Thomas Berer, Thomas Berer, } "Frequency-domain photoacoustic and fluorescence microscopy: application on labeled and unlabeled cells", Proc. SPIE 10487, Multimodal Biomedical Imaging XIII, 1048709 (13 February 2018); doi: 10.1117/12.2290023; https://doi.org/10.1117/12.2290023

Back to Top