14 February 2018 Resonant nanopillars as label-free optical biosensors
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In recent works it has been demonstrated the suitability of using resonant nanopillars (R-NPs) as biochemical. In this work it has been shown the capability of the R-NPs to behave as label-free multiplexed biological sensors. Each R-NP is formed by silicon oxide (SiO2) and silicon nitride (Si3N4) Bragg reflectors and a central cavity of SiO2, and they are grouped into eight arrays called BICELLs, which are distributed on a single chip of quartz substrate for multiplexing measurements. For the biological sensing assessment it was developed an immunoassay on the eight single BICELLs. The biofunctionalization process was performed by a silanization protocol based on 3-aminopropyltrymethoxysilane (APTMS) and glutaradheyde (GA) as a linker between APTMS and the IgG which acted as biorreceptor for the anti-IgG recognition. In this work, there were compared two forms of immobilization: on one hand by incubating the R-NPs under static drop of 50 μg/mL and on the second hand by introducing the sensing chip in a flow cell with a continuous flow of the same concentration of IgG. The eight arrays of R-NPs or BICELLs were independently optically interrogated by a bundle of fiber connected to a spectrometer. The multiplexing analysis showed reproducibility among the BICELLs, suggesting the potentially of using R-NPs for multiplexed biosensors. Performance in the immobilization process apparently does not have a signification effect. However the election of one method or another should be a commitment between time and resources.
Conference Presentation
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Ana López-Hernandez, Ana López-Hernandez, Rafael Casquel, Rafael Casquel, Miguel Holgado , Miguel Holgado , Iñaki Cornago, Iñaki Cornago, Fátima Fernández, Fátima Fernández, Paula Ciaurriz, Paula Ciaurriz, Francisco J. Sanza, Francisco J. Sanza, Beatriz Santamaría, Beatriz Santamaría, Maria V. Maigler, Maria V. Maigler, María Fe Laguna, María Fe Laguna, } "Resonant nanopillars as label-free optical biosensors", Proc. SPIE 10488, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVIII, 1048814 (14 February 2018); doi: 10.1117/12.2288273; https://doi.org/10.1117/12.2288273

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