Stokes shift spectroscopy for the early diagnosis of epithelial precancers in DMBA treated mouse skin carcinogenesis

Ebenezar Jeyasingh; Ganesan Singaravelu; Aruna Prakasarao

doi:10.1117/12.2287486

21 February 2018 Stokes shift spectroscopy for the early diagnosis of epithelial precancers in DMBA treated mouse skin carcinogenesis

Ebenezar Jeyasingh, Ganesan Singaravelu, Aruna Prakasarao

Author Affiliations +

Proceedings Volume 10489, Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis; 1048917 (2018) https://doi.org/10.1117/12.2287486
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

In this study, we aim to characterize the tissue transformation in dimethylbenz(a)anthracene (DMBA) treated mouse skin tumor model using stokes shift spectroscopy (SSS) technique for early detection of the neoplastic changes. Stokes shift (SS) spectra measured by scanning both excitation and emission wavelength simultaneously with a fixed wavelength of interval (Δλ=20 nm) in vivo from 33 DMBA treated animals and 6 control animals. The SS spectra of normal (n=6), hyperplasia (n=10), dysplasia (n=10), and WDSCC (n=13) of mice skin shows the distinct peaks around 300, 350, and 386 nm may be attributed to tryptophan, collagen, and NADH respectively. From the observed spectral differences and the ratio variables that resulted in better classification between groups, it is concluded that tryptophan, collagen, and NADH are the key fluorophores that undergo changes during tissue transformation process and hence they can be targeted as tumor markers for early neoplastic changes.

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Ebenezar Jeyasingh, Ganesan Singaravelu, and Aruna Prakasarao "Stokes shift spectroscopy for the early diagnosis of epithelial precancers in DMBA treated mouse skin carcinogenesis", Proc. SPIE 10489, Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis, 1048917 (21 February 2018); https://doi.org/10.1117/12.2287486

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KEYWORDS

Skin

Tissues

Collagen

Cancer

Diagnostics

Spectroscopy

Tumors

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