21 February 2018 Intrinsic fluorescence based in-vivo detection of cervical precancer with hand held prototype device
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A prototype device (hand held probe) designed and fabricated in the lab has been tested for cervical precancer detection using intrinsic fluorescence. The intrinsic fluorescence gets strongly modulated by the interplay of scattering and absorption. This masks valuable biochemical information which is present in the intrinsic fluorescence. These distortion effects can be minimized by normalizing the polarized fluorescence spectra by the polarized elastic scattering spectra. The measurements have been made with a in-house fabricated device using a 405 nm diode laser and white light source respectively. 166 sites of different grades of cervical pre-cancer biopsy samples (CIN I and CIN II) (CIN: cervical intraepithelial neoplastic) have been discriminated from 29 sites of normal biopsy samples using principal component analysis (PCA) based linear discriminant analysis (LDA). The sensitivity and specificity for discrimination of normal samples from CIN I are found to be 99% and 96% respectively. Further the normal samples can be discriminated from CIN II samples with 96% sensitivity and 96% specificity. Based on these promising ex-vivo results an in-vivo study on patients has been initiated in the hospital. The hand held device built in-house shows promise as a useful tool for in vivo cervical precancer detection by polarized fluorescence. Preliminary in-vivo results on 10 patients indicate the efficacy of the hand held device for screening cervical precancers using intrinsic fluorescence.
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Bharat Lal Meena, Bharat Lal Meena, Akanksha Raikwar, Akanksha Raikwar, Kiran Pandey, Kiran Pandey, Asha Agarwal, Asha Agarwal, Chayanika Pantola, Chayanika Pantola, Asima Pradhan, Asima Pradhan, } "Intrinsic fluorescence based in-vivo detection of cervical precancer with hand held prototype device", Proc. SPIE 10489, Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis, 104891G (21 February 2018); doi: 10.1117/12.2289911; https://doi.org/10.1117/12.2289911


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