21 March 2018 Dual Raman-Brillouin spectroscopic investigation of plant stress response and development
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Raman and Brillouin spectroscopy are powerful tools for non-invasive and non-destructive investigations of material chemical and mechanical properties. In this study, we use a newly developed custom-built dual Raman-Brillouin microspectroscopy instrument to build on previous works studying in-vivo stress response of live plants using only a Raman spectroscopy system. This dual Raman-Brillouin spectroscopy system is capable of fast simultaneous spectra acquisition from single-point locations. Shifts and changes in a samples Brillouin spectrum indicate a change in the physical characteristics of the sample, namely mechano-elasticity; in measuring this change, we can establish a relationship between the mechanical properties of a sample and known stress response agents, such as reactive oxygen species and other chemical constituents as indicated by peaks in the Raman spectra of the same acquisition point. Simultaneous application of these spectroscopic techniques offers great promise for future development and applications in agricultural and biological studies and can help to improve our understanding of mechanochemical changes of plants and other biological samples in response to environmental and chemically induced stresses at microscopic or cellular level.
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Zachary Coker, Zachary Coker, Maria Troyanova-Wood, Maria Troyanova-Wood, Narangerel Altangerel, Narangerel Altangerel, Kassie Marble, Kassie Marble, Vladislav Yakovlev, Vladislav Yakovlev, } "Dual Raman-Brillouin spectroscopic investigation of plant stress response and development", Proc. SPIE 10490, Biomedical Vibrational Spectroscopy 2018: Advances in Research and Industry, 104900I (21 March 2018); doi: 10.1117/12.2291842; https://doi.org/10.1117/12.2291842

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