19 February 2018 Pathology in a tube step 2: simple rapid fabrication of curved circular cross section millifluidic channels for biopsy preparation/3D imaging towards pancreatic cancer detection and diagnosis
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Abstract
3D pathology is intrinsically dependent on 3D microscopy, or the whole tissue imaging of patient tissue biopsies (TBs). Consequently, unsectioned needle specimens must be processed whole: a procedure which cannot necessarily be accomplished through manual methods, or by retasking automated pathology machines. Thus "millifluidic" devices (for millimeter-scale biopsies) are an ideal solution for tissue handling/preparation. TBs are large, messy and a solid-liquid mixture; they vary in material, geometry and structure based on the organ biopsied, the clinician skill and the needle type used. As a result, traditional microfluidic devices are insufficient to handle such mm-sized samples and their associated fabrication techniques are impractical and costly with respect to time/efficiency. Our research group has devised a simple, rapid fabrication process for millifluidic devices using jointed skeletal molds composed of machined, reusable metal rods, segmented rods and stranded wire as structural cores; these cores are surrounded by Teflon outer housing. We can therefore produce curving, circular-cross-section (CCCS) millifluidic channels in rapid fashion that cannot normally be achieved by microfabrication, micro-/CNC-machining, or 3D printing. The approach has several advantages. CLINICAL: round channels interface coring needles. PROCESSING: CCCS channels permit multi-layer device designs for additional (processing, monitoring, testing) stages. REUSABILITY: for a biopsy/needle diameter, molding (interchangeable) components may be produced one-time then reused for other designs. RAPID: structural cores can be quickly removed due to Teflon®'s ultra-low friction; housing may be released with ethanol; PDMS volumes cure faster since metal skeleton molds conduct additional heat from within the curing elastomer.
Conference Presentation
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Ronnie Das, Ronnie Das, Chris W. Burfeind, Chris W. Burfeind, Saniel D. Lim, Saniel D. Lim, Shubham Patle, Shubham Patle, Eric J. Seibel, Eric J. Seibel, } "Pathology in a tube step 2: simple rapid fabrication of curved circular cross section millifluidic channels for biopsy preparation/3D imaging towards pancreatic cancer detection and diagnosis", Proc. SPIE 10491, Microfluidics, BioMEMS, and Medical Microsystems XVI, 1049118 (19 February 2018); doi: 10.1117/12.2291018; https://doi.org/10.1117/12.2291018
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