13 February 2018 Optical coefficient measurements using bulk living tissue by an optical fiber puncture with FOV change
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To avoid an instability of the optical coefficient measurement using sliced tissue preparation, we proposed the combination of light intensity measurement through an optical fiber puncturing into a bulk tissue varying field of view (FOV) and ray tracing calculation using Monte-Carlo method. The optical coefficients of myocardium such as absorption coefficient μa, scattering coefficient μs, and anisotropic parameter g are used in the myocardium optical propagation. Since optical coefficients obtained using thin sliced tissue could be instable because they are affected by dehydration and intracellular fluid effusion on the sample surface, variety of coefficients have been reported over individual optical differences of living samples.

The proposed method which combined the experiment using the bulk tissue with ray tracing calculation were performed. In this method, a 200 μmΦ high-NA silica fiber installed in a 21G needle was punctured up to the bottom of the myocardial bulk tissue over 3 cm in thickness to measure light intensity changing the fiber-tip depth and FOV. We found that the measured attenuation coefficients decreased as the FOV increased. The ray trace calculation represented the same FOV dependence in above mentioned experimental result. We think our particular fiber punctured measurement using bulk tissue varying FOV with Inverse Monte-Carlo method might be useful to obtain the optical coefficients to avoid sample preparation instabilities.
Conference Presentation
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Haruna Nakazawa, Haruna Nakazawa, Marika Doi, Marika Doi, Emiyu Ogawa, Emiyu Ogawa, Tsunenori Arai, Tsunenori Arai, } "Optical coefficient measurements using bulk living tissue by an optical fiber puncture with FOV change", Proc. SPIE 10492, Optical Interactions with Tissue and Cells XXIX, 104920P (13 February 2018); doi: 10.1117/12.2289332; https://doi.org/10.1117/12.2289332

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