13 February 2018 Deep-tissue two-photon imaging in brain and peripheral nerve with a compact high-pulse energy ytterbium fiber laser
Author Affiliations +
Two-photon microscopy is a powerful tool of current scientific research, allowing optical visualization of structures below the surface of tissues. This is of particular value in neuroscience, where optically accessing regions within the brain is critical for the continued advancement in understanding of neural circuits. However, two-photon imaging at significant depths have typically used Ti:Sapphire based amplifiers that are prohibitively expensive and bulky. In this study, we demonstrate deep tissue two-photon imaging using a compact, inexpensive, turnkey operated Ytterbium fiber laser (Y-Fi, KM Labs). The laser is based on all-normal dispersion (ANDi) that provides short pulse durations and high pulse energies. Depth measurements obtained in ex vivo mouse cortex exceed those obtainable with standard two-photon microscopes using Ti:Sapphire lasers. In addition to demonstrating the capability of deep-tissue imaging in the brain, we investigated imaging depth in highly-scattering white matter with measurements in sciatic nerve showing limited optical penetration of heavily myelinated nerve tissue relative to grey matter.
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Arjun K. Fontaine, Arjun K. Fontaine, Matthew S. Kirchner, Matthew S. Kirchner, John H. Caldwell, John H. Caldwell, Richard F. Weir, Richard F. Weir, Emily A. Gibson, Emily A. Gibson, "Deep-tissue two-photon imaging in brain and peripheral nerve with a compact high-pulse energy ytterbium fiber laser", Proc. SPIE 10492, Optical Interactions with Tissue and Cells XXIX, 1049217 (13 February 2018); doi: 10.1117/12.2309490; https://doi.org/10.1117/12.2309490


Resolution degradation due to brain tissue scattering
Proceedings of SPIE (April 30 1998)
Laser imaging for clinical applications
Proceedings of SPIE (March 29 1995)

Back to Top