Real-time volumetric mapping of calcium activity in living mice by functional optoacoustic neuro-tomography (Conference Presentation)

Sven Gottschalk; Xose Luis Deán-Ben; Shy Shoham; Daniel Razansky

doi:10.1117/12.2289685

15 March 2018 Real-time volumetric mapping of calcium activity in living mice by functional optoacoustic neuro-tomography (Conference Presentation)

Sven Gottschalk, Xose Luis Deán-Ben, Shy Shoham, Daniel Razansky

Author Affiliations +

Proceedings Volume 10494, Photons Plus Ultrasound: Imaging and Sensing 2018; 104941I (2018) https://doi.org/10.1117/12.2289685
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Understanding brain functionality remains an arduous task despite decades of research employing a wide array of neural recording and imaging techniques. Genetically encoded calcium indicators (GECIs) are powerful and versatile tools for tagging fast neural activity in a large number of neurons with excellent spatial localization. Here we demonstrate that functional optoacoustic neuro-tomography (FONT) enables non-invasive imaging of sensory-evoked activity in GCaMP6-expressing mouse brain in vivo, thus holding promise for large-scale neural recording at penetration depths and spatio-temporal resolution scales not covered with the existing neuroimaging techniques. The FONT imaging system has volumetric temporal resolution of 10msec and spatial resolution of 150µm across an effective field of view of 2cm3 covering an entire mouse brain. However, the effective depth in the current study was restricted to the cortical areas due to the limited penetration of light at the visible wavelengths used for the GCaMP6 excitation. The stimulation protocol involving somatosensory electrical hindpaw stimulation was specifically designed to minimize the influence of hemodynamic changes indirectly associated with neuronal activity, which are much slower than the GCaMP-related calcium transients. Rapid optoacoustic signal transients were observed in the activated brain regions but not inside major blood vessels, thus allowing for a clear differentiation of the calcium-related activity from the underlying hemodynamic responses. Our study is the first to examine fast volumetric optoacoustic signatures of GECIs non-invasively in living mice, further showing that the corresponding changes of their fluorescence are directly related to the optoacoustic responses.

Conference Presentation

Citation Download Citation

Sven Gottschalk, Xose Luis Deán-Ben, Shy Shoham, and Daniel Razansky "Real-time volumetric mapping of calcium activity in living mice by functional optoacoustic neuro-tomography (Conference Presentation)", Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 104941I (15 March 2018); https://doi.org/10.1117/12.2289685

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available