19 February 2018 Photoacoustic microscopy of single cells employing an intensity-modulated diode laser
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Abstract
In this work, we employ frequency-domain photoacoustic microscopy to obtain photoacoustic images of labeled and unlabeled cells. The photoacoustic microscope is based on an intensity-modulated diode laser in combination with a focused piezo-composite transducer and allows imaging of labeled cells without severe photo-bleaching. We demonstrate that frequency-domain photoacoustic microscopy realized with a diode laser is capable of recording photoacoustic images of single cells with sub-µm resolution. As examples, we present images of undyed human red blood cells, stained human epithelial cells, and stained yeast cells.
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Gregor Langer, Bianca Buchegger, Jaroslaw Jacak, Manoj Kumar Dasa, Thomas A. Klar, Thomas Berer, "Photoacoustic microscopy of single cells employing an intensity-modulated diode laser", Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 104942L (19 February 2018); doi: 10.1117/12.2289978; https://doi.org/10.1117/12.2289978
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