19 February 2018 Intravital hybrid optical-optoacoustic microscopy based on fiber-Bragg interferometry
Author Affiliations +
Optoacoustic microscopy (OAM) has enabled high-resolution, label-free imaging of tissues at depths not achievable with purely optical microscopy. However, widespread implementation of OAM into existing epi-illumination microscopy setups is often constrained by the performance and size of the commonly used piezoelectric ultrasound detectors. In this work, we introduce a novel acoustic detector based on a π-phase-shifted fiber Bragg grating (π-FBG) interferometer embedded inside an ellipsoidal acoustic cavity. The cavity enables seamless integration of epi-illumination OAM into existing microscopy setups by decoupling the acoustic and optical paths between the microscope objective and the sample. The cavity also acts as an acoustic condenser, boosting the sensitivity of the π-FBG and enabling cost effective CW-laser interrogation technique. We characterize the sensor’s sensitivity and bandwidth and demonstrate hybrid OAM and second-harmonic imaging of phantoms and mouse tissue in vivo.
Conference Presentation
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Rami Shnaiderman, Rami Shnaiderman, Georg Wissmeyer, Georg Wissmeyer, Markus Seeger, Markus Seeger, Hector Estrada, Hector Estrada, Vasilis Ntziachristos, Vasilis Ntziachristos, } "Intravital hybrid optical-optoacoustic microscopy based on fiber-Bragg interferometry ", Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 104942M (19 February 2018); doi: 10.1117/12.2290281; https://doi.org/10.1117/12.2290281

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