19 February 2018 Integrated photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy for multimodal chorioretinal imaging
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Abstract
Current clinical available retinal imaging techniques have limitations, including limited depth of penetration or requirement for the invasive injection of exogenous contrast agents. Here, we developed a novel multimodal imaging system for high-speed, high-resolution retinal imaging of larger animals, such as rabbits. The system integrates three state-of-the-art imaging modalities, including photoacoustic microscopy (PAM), optical coherence tomography (OCT), and fluorescence microscopy (FM). In vivo experimental results of rabbit eyes show that the PAM is able to visualize laser-induced retinal burns and distinguish individual eye blood vessels using a laser exposure dose of ~80 nJ, which is well below the American National Standards Institute (ANSI) safety limit 160 nJ. The OCT can discern different retinal layers and visualize laser burns and choroidal detachments. The novel multi-modal imaging platform holds great promise in ophthalmic imaging.
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Chao Tian, Chao Tian, Wei Zhang, Wei Zhang, Van Phuc Nguyen, Van Phuc Nguyen, Ziyi Huang, Ziyi Huang, Xueding Wang, Xueding Wang, Yannis M. Paulus, Yannis M. Paulus, } "Integrated photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy for multimodal chorioretinal imaging", Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 104945U (19 February 2018); doi: 10.1117/12.2290594; https://doi.org/10.1117/12.2290594
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