20 February 2018 Applications and assessment of an excitation-scanning hyperspectral imaging system
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The majority of microscopic and endoscopic technologies utilize white light illumination. For a number of applications, hyper-spectral imaging can be shown to have significant improvements over standard white-light imaging techniques. This is true for both microscopy and in vivo imaging. However, hyperspectral imaging methods have suffered from slow application times. Often, minutes are required to gather a full imaging stack. Here we will describe and evaluate a novel excitation-scanning hyperspectral imaging system and discuss some applications. We have developed and are optimizing a novel approach called excitation-scanning hyperspectral imaging that provides an order of magnitude increased signal strength. This excitation scanning technique has enabled us to produce a microscopy system capable of high speed hyperspectral imaging with the potential for live video acquisition. The excitation-scanning hyperspectral imaging technology we developed may impact a range of applications. The current design uses digital strobing to illuminate at 16 wavelengths with millisecond image acquisition time. Analog intensity control enables a fully customizable excitation profile. A significant advantage of excitation-scanning hyperspectral imaging is can identify multiple targets simultaneously in real time. Finally, we are exploring utilizing this technology for a variety of applications ranging from measuring cAMP distribution in three dimensions within a cell to electrophysiology.
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Sam A. Mayes, Sam A. Mayes, Kaysie Moore, Kaysie Moore, Craig Browning, Craig Browning, Phiwat Klomkaew, Phiwat Klomkaew, Thomas C. Rich, Thomas C. Rich, Silas J. Leavesley, Silas J. Leavesley, "Applications and assessment of an excitation-scanning hyperspectral imaging system", Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 1049706 (20 February 2018); doi: 10.1117/12.2290120; https://doi.org/10.1117/12.2290120

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