Miniaturized microscope for high throughput screening of tumor spheroids in microfluidic devices

Javier Uranga; Alejandro Rodríguez-Pena; Desiré Gahigiro; Carlos Ortiz-de-Solorzano

doi:10.1117/12.2287668

20 February 2018 Miniaturized microscope for high throughput screening of tumor spheroids in microfluidic devices

Javier Uranga, Alejandro Rodríguez-Pena, Desiré Gahigiro, Carlos Ortiz-de-Solorzano

Proceedings Volume 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI; 104970A (2018) https://doi.org/10.1117/12.2287668
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

High-throughput in vitro screening of highly physiological three-dimensional cell cultures (3D-HTS) is rapidly gaining importance in preclinical studies, to study the effect of the microenvironment in tumor development, and to evaluate the efficacy of new anticancer drugs. Furthermore, it could also be envisioned the use of 3D-HTS systems in personalized anti-cancer treatment planning, based on tumor organoids or spheroids grown from tumor biopsies or isolated tumor circulating cells. Most commercial, multi-well plate based 3D-HTS systems are large, expensive, and are based on the use of multi-well plates that hardly provide a physiological environment and require the use of large amounts of biological material and reagents. In this paper we present a novel, miniaturized inverted microscope (hereinafter miniscospe), made up of low-cost, mass producible parts, that can be used to monitor the growth of living tumor cell spheroids within customized three-dimensional microfluidic platforms. Our 3D-HTS miniscope combines phase contrast imaging based on oblique back illumination technique with traditional widefield epi-fluorescence imaging, implemented using miniaturized electro-optical parts and gradient-index refraction lenses. This small (3x6x2cm), lightweight device can effectively image overtime the growth of (>200) tumor spheroids in a controlled and reproducible environment. Our miniscope can be used to acquire time-lapse images of cellular living spheroids over the course of several hours and captures their growth before and after drug treatment, to evaluate the effectiveness of the drug.

Conference Presentation

Citation Download Citation

Javier Uranga, Alejandro Rodríguez-Pena, Desiré Gahigiro, and Carlos Ortiz-de-Solorzano "Miniaturized microscope for high throughput screening of tumor spheroids in microfluidic devices", Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 104970A (20 February 2018); https://doi.org/10.1117/12.2287668

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available