Label-free cell viability assay using lens-free microscopy

C. Allier; T. Bordy; L. Hervé; O. Cioni; G. Esteban; M. Pisaneschi; S. Morales

doi:10.1117/12.2289811

20 February 2018 Label-free cell viability assay using lens-free microscopy

C. Allier, T. Bordy, L. Hervé, O. Cioni, G. Esteban, M. Pisaneschi, S. Morales

Proceedings Volume 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI; 104970B (2018) https://doi.org/10.1117/12.2289811
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

We introduce a label-free technique based on lens-free microscopy to perform cell counting and cell viability assay. Without the use of any labelling, the discrimination between alive and dead cells is obtained by analyzing the cells on the basis of their holographic signature. We have assessed this novel technique by comparing the obtained results in terms of viability and cell counting against automatic optical counting and trypan blue staining as reference methods. The lensfree measurements agree very well with the reference techniques up to ~30.10⁶ cells/ml. We found a coefficient of determination R² of 0.99 and a slope of 1.01 for the viability measurements (N=84 CHO cell samples).

Conference Presentation

Citation Download Citation

C. Allier, T. Bordy, L. Hervé, O. Cioni, G. Esteban, M. Pisaneschi, and S. Morales "Label-free cell viability assay using lens-free microscopy", Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 104970B (20 February 2018); https://doi.org/10.1117/12.2289811

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