Fluorescence lifetime microscopy for monitoring cell adhesion using metal induced energy transfer

Wonsang Hwang; JinWon Seo; Jun ho Song; DongEun Kim; YoungJae Won; In-Hong Choi; Kyung-Hwa Yoo; Dug Young Kim

doi:10.1117/12.2289330

20 February 2018 Fluorescence lifetime microscopy for monitoring cell adhesion using metal induced energy transfer

Wonsang Hwang; JinWon Seo; Jun ho Song; DongEun Kim; YoungJae Won; In-Hong Choi; Kyung-Hwa Yoo; Dug Young Kim

Author Affiliations +

Proceedings Volume 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI; 104970E (2018) https://doi.org/10.1117/12.2289330
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

A precise control and a reliable monitoring tool for the adhesion properties of a cell are very important in atherosclerosis studies. If endothelial cells in contact with the intracellular membrane are not attached securely, low-density lipoprotein (LDL) particles can enter into the inner membrane. It is therefore necessary to measure conditions under which endothelial cell detachment occurs. When a cell is attached to a metal thin film, the lifetime of a fluorescence probe attached to the membrane of the cell is reduced by the metal-induced energy transfer (MIET). Fluorescence lifetime imaging microscopy (FLIM) is used to monitor the attachment condition of a cell to a metal surface using FRET. However, this requires high numerical aperture (NA) objective lens because axial confocal resolution must be smaller than the cell thickness. This requirement limits the field of view of the measurement specimen. In this study we provides a new method which can measure adhesion properties of endothelial cells even with a low NA objective lens by resolving two lifetime components in FLIM.

Citation Download Citation

Wonsang Hwang, Wonsang Hwang, JinWon Seo, JinWon Seo, Jun ho Song, Jun ho Song, DongEun Kim, DongEun Kim, YoungJae Won, YoungJae Won, In-Hong Choi, In-Hong Choi, Kyung-Hwa Yoo, Kyung-Hwa Yoo, Dug Young Kim, Dug Young Kim, } "Fluorescence lifetime microscopy for monitoring cell adhesion using metal induced energy transfer", Proc. SPIE 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI, 104970E (20 February 2018); doi: 10.1117/12.2289330; https://doi.org/10.1117/12.2289330

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